Review



3d cell culture coating kit  (Beyotime)


Bioz Verified Symbol Beyotime is a verified supplier
Bioz Manufacturer Symbol Beyotime manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 99
      Buy from Supplier

    Structured Review

    Beyotime 3d cell culture coating kit
    Cellular uptake and <t>tumor</t> <t>spheroid</t> penetration. (A) Confocal microscopy images of CT26-luc cells after 2 h of incubation with C6@LF NPs, C6@LFHA NPs, and competitive inhibition groups pre-treated with free LF or HA. Scale bar: 50 μm. (B) Flow cytometry histograms of (i) CT26-luc cells, (ii) human umbilical vein endothelial cells (HUVECs), and (iii) M2-polarized bone marrow-derived macrophages (M2-BMDMs) treated with C6@LF NPs, C6@LFHA NPs, and competitive inhibition groups. (C) Lysosomal co-localization of C6@LFHA NPs in HUVECs after 8 h incubation. Quantitative analysis showed a Pearson's correlation coefficient of 0.34 ± 0.026. (D) Penetration of C6@LFHA NPs into CT26-luc tumor spheroids, as shown by Z-stack slices (Scale bar: 20 μm), maximum intensity projection, and <t>3D</t> reconstruction (generated using ImageJ). Scale bar: 200 μm. All data are presented as mean ± SD (n = 3).
    3d Cell Culture Coating Kit, supplied by Beyotime, used in various techniques. Bioz Stars score: 99/100, based on 23 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/3d+cell+culture+coating+kit/pmc12805111-94-14-19?v=Beyotime
    Average 99 stars, based on 23 article reviews
    3d cell culture coating kit - by Bioz Stars, 2026-08
    99/100 stars

    Images

    1) Product Images from "LRP-1/CD44-targeted regorafenib nano-delivery system leveraging anti-angiogenesis and synergistic cytotoxicity against peritoneal metastasis of colorectal cancer"

    Article Title: LRP-1/CD44-targeted regorafenib nano-delivery system leveraging anti-angiogenesis and synergistic cytotoxicity against peritoneal metastasis of colorectal cancer

    Journal: Bioactive Materials

    doi: 10.1016/j.bioactmat.2025.12.015

    Cellular uptake and tumor spheroid penetration. (A) Confocal microscopy images of CT26-luc cells after 2 h of incubation with C6@LF NPs, C6@LFHA NPs, and competitive inhibition groups pre-treated with free LF or HA. Scale bar: 50 μm. (B) Flow cytometry histograms of (i) CT26-luc cells, (ii) human umbilical vein endothelial cells (HUVECs), and (iii) M2-polarized bone marrow-derived macrophages (M2-BMDMs) treated with C6@LF NPs, C6@LFHA NPs, and competitive inhibition groups. (C) Lysosomal co-localization of C6@LFHA NPs in HUVECs after 8 h incubation. Quantitative analysis showed a Pearson's correlation coefficient of 0.34 ± 0.026. (D) Penetration of C6@LFHA NPs into CT26-luc tumor spheroids, as shown by Z-stack slices (Scale bar: 20 μm), maximum intensity projection, and 3D reconstruction (generated using ImageJ). Scale bar: 200 μm. All data are presented as mean ± SD (n = 3).
    Figure Legend Snippet: Cellular uptake and tumor spheroid penetration. (A) Confocal microscopy images of CT26-luc cells after 2 h of incubation with C6@LF NPs, C6@LFHA NPs, and competitive inhibition groups pre-treated with free LF or HA. Scale bar: 50 μm. (B) Flow cytometry histograms of (i) CT26-luc cells, (ii) human umbilical vein endothelial cells (HUVECs), and (iii) M2-polarized bone marrow-derived macrophages (M2-BMDMs) treated with C6@LF NPs, C6@LFHA NPs, and competitive inhibition groups. (C) Lysosomal co-localization of C6@LFHA NPs in HUVECs after 8 h incubation. Quantitative analysis showed a Pearson's correlation coefficient of 0.34 ± 0.026. (D) Penetration of C6@LFHA NPs into CT26-luc tumor spheroids, as shown by Z-stack slices (Scale bar: 20 μm), maximum intensity projection, and 3D reconstruction (generated using ImageJ). Scale bar: 200 μm. All data are presented as mean ± SD (n = 3).

    Techniques Used: Confocal Microscopy, Incubation, Inhibition, Flow Cytometry, Derivative Assay, Generated



    Similar Products

    3d cell culture coating kit  (Beyotime)
    99
    Beyotime 3d cell culture coating kit
    Cellular uptake and <t>tumor</t> <t>spheroid</t> penetration. (A) Confocal microscopy images of CT26-luc cells after 2 h of incubation with C6@LF NPs, C6@LFHA NPs, and competitive inhibition groups pre-treated with free LF or HA. Scale bar: 50 μm. (B) Flow cytometry histograms of (i) CT26-luc cells, (ii) human umbilical vein endothelial cells (HUVECs), and (iii) M2-polarized bone marrow-derived macrophages (M2-BMDMs) treated with C6@LF NPs, C6@LFHA NPs, and competitive inhibition groups. (C) Lysosomal co-localization of C6@LFHA NPs in HUVECs after 8 h incubation. Quantitative analysis showed a Pearson's correlation coefficient of 0.34 ± 0.026. (D) Penetration of C6@LFHA NPs into CT26-luc tumor spheroids, as shown by Z-stack slices (Scale bar: 20 μm), maximum intensity projection, and <t>3D</t> reconstruction (generated using ImageJ). Scale bar: 200 μm. All data are presented as mean ± SD (n = 3).
    3d Cell Culture Coating Kit, supplied by Beyotime, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/3d+cell+culture+coating+kit/pmc12805111-94-14-19?v=Beyotime
    Average 99 stars, based on 1 article reviews
    3d cell culture coating kit - by Bioz Stars, 2026-08
    99/100 stars
    		  Buy from Supplier
    3d cell culture envelope kit  (Beyotime)
    99
    Beyotime 3d cell culture envelope kit
    Cellular uptake and <t>tumor</t> <t>spheroid</t> penetration. (A) Confocal microscopy images of CT26-luc cells after 2 h of incubation with C6@LF NPs, C6@LFHA NPs, and competitive inhibition groups pre-treated with free LF or HA. Scale bar: 50 μm. (B) Flow cytometry histograms of (i) CT26-luc cells, (ii) human umbilical vein endothelial cells (HUVECs), and (iii) M2-polarized bone marrow-derived macrophages (M2-BMDMs) treated with C6@LF NPs, C6@LFHA NPs, and competitive inhibition groups. (C) Lysosomal co-localization of C6@LFHA NPs in HUVECs after 8 h incubation. Quantitative analysis showed a Pearson's correlation coefficient of 0.34 ± 0.026. (D) Penetration of C6@LFHA NPs into CT26-luc tumor spheroids, as shown by Z-stack slices (Scale bar: 20 μm), maximum intensity projection, and <t>3D</t> reconstruction (generated using ImageJ). Scale bar: 200 μm. All data are presented as mean ± SD (n = 3).
    3d Cell Culture Envelope Kit, supplied by Beyotime, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/3d+cell+culture+coating+kit/pmc12619588-65-6-14?v=Beyotime
    Average 99 stars, based on 1 article reviews
    3d cell culture envelope kit - by Bioz Stars, 2026-08
    99/100 stars
    		  Buy from Supplier
    well cell culture plates  (Beyotime)
    99
    Beyotime well cell culture plates
    Cellular uptake and <t>tumor</t> <t>spheroid</t> penetration. (A) Confocal microscopy images of CT26-luc cells after 2 h of incubation with C6@LF NPs, C6@LFHA NPs, and competitive inhibition groups pre-treated with free LF or HA. Scale bar: 50 μm. (B) Flow cytometry histograms of (i) CT26-luc cells, (ii) human umbilical vein endothelial cells (HUVECs), and (iii) M2-polarized bone marrow-derived macrophages (M2-BMDMs) treated with C6@LF NPs, C6@LFHA NPs, and competitive inhibition groups. (C) Lysosomal co-localization of C6@LFHA NPs in HUVECs after 8 h incubation. Quantitative analysis showed a Pearson's correlation coefficient of 0.34 ± 0.026. (D) Penetration of C6@LFHA NPs into CT26-luc tumor spheroids, as shown by Z-stack slices (Scale bar: 20 μm), maximum intensity projection, and <t>3D</t> reconstruction (generated using ImageJ). Scale bar: 200 μm. All data are presented as mean ± SD (n = 3).
    Well Cell Culture Plates, supplied by Beyotime, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/3d+cell+culture+coating+kit/pmc12218950-182-12-21?v=Beyotime
    Average 99 stars, based on 1 article reviews
    well cell culture plates - by Bioz Stars, 2026-08
    99/100 stars
    		  Buy from Supplier
    96 well round bottom plate coatedwith 3d cell culture coating solution  (Beyotime)
    99
    Beyotime 96 well round bottom plate coatedwith 3d cell culture coating solution
    Cellular uptake and <t>tumor</t> <t>spheroid</t> penetration. (A) Confocal microscopy images of CT26-luc cells after 2 h of incubation with C6@LF NPs, C6@LFHA NPs, and competitive inhibition groups pre-treated with free LF or HA. Scale bar: 50 μm. (B) Flow cytometry histograms of (i) CT26-luc cells, (ii) human umbilical vein endothelial cells (HUVECs), and (iii) M2-polarized bone marrow-derived macrophages (M2-BMDMs) treated with C6@LF NPs, C6@LFHA NPs, and competitive inhibition groups. (C) Lysosomal co-localization of C6@LFHA NPs in HUVECs after 8 h incubation. Quantitative analysis showed a Pearson's correlation coefficient of 0.34 ± 0.026. (D) Penetration of C6@LFHA NPs into CT26-luc tumor spheroids, as shown by Z-stack slices (Scale bar: 20 μm), maximum intensity projection, and <t>3D</t> reconstruction (generated using ImageJ). Scale bar: 200 μm. All data are presented as mean ± SD (n = 3).
    96 Well Round Bottom Plate Coatedwith 3d Cell Culture Coating Solution, supplied by Beyotime, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/3d+cell+culture+coating+kit/pm40335474-253-16-25?v=Beyotime
    Average 99 stars, based on 1 article reviews
    96 well round bottom plate coatedwith 3d cell culture coating solution - by Bioz Stars, 2026-08
    99/100 stars
    		  Buy from Supplier
    well round bottom plate  (Beyotime)
    99
    Beyotime well round bottom plate
    Cellular uptake and <t>tumor</t> <t>spheroid</t> penetration. (A) Confocal microscopy images of CT26-luc cells after 2 h of incubation with C6@LF NPs, C6@LFHA NPs, and competitive inhibition groups pre-treated with free LF or HA. Scale bar: 50 μm. (B) Flow cytometry histograms of (i) CT26-luc cells, (ii) human umbilical vein endothelial cells (HUVECs), and (iii) M2-polarized bone marrow-derived macrophages (M2-BMDMs) treated with C6@LF NPs, C6@LFHA NPs, and competitive inhibition groups. (C) Lysosomal co-localization of C6@LFHA NPs in HUVECs after 8 h incubation. Quantitative analysis showed a Pearson's correlation coefficient of 0.34 ± 0.026. (D) Penetration of C6@LFHA NPs into CT26-luc tumor spheroids, as shown by Z-stack slices (Scale bar: 20 μm), maximum intensity projection, and <t>3D</t> reconstruction (generated using ImageJ). Scale bar: 200 μm. All data are presented as mean ± SD (n = 3).
    Well Round Bottom Plate, supplied by Beyotime, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/3d+cell+culture+coating+kit/pmc12058976-260-17-27?v=Beyotime
    Average 99 stars, based on 1 article reviews
    well round bottom plate - by Bioz Stars, 2026-08
    99/100 stars
    		  Buy from Supplier
    round bottom 96 well plate  (Beyotime)
    99
    Beyotime round bottom 96 well plate
    Cellular uptake and <t>tumor</t> <t>spheroid</t> penetration. (A) Confocal microscopy images of CT26-luc cells after 2 h of incubation with C6@LF NPs, C6@LFHA NPs, and competitive inhibition groups pre-treated with free LF or HA. Scale bar: 50 μm. (B) Flow cytometry histograms of (i) CT26-luc cells, (ii) human umbilical vein endothelial cells (HUVECs), and (iii) M2-polarized bone marrow-derived macrophages (M2-BMDMs) treated with C6@LF NPs, C6@LFHA NPs, and competitive inhibition groups. (C) Lysosomal co-localization of C6@LFHA NPs in HUVECs after 8 h incubation. Quantitative analysis showed a Pearson's correlation coefficient of 0.34 ± 0.026. (D) Penetration of C6@LFHA NPs into CT26-luc tumor spheroids, as shown by Z-stack slices (Scale bar: 20 μm), maximum intensity projection, and <t>3D</t> reconstruction (generated using ImageJ). Scale bar: 200 μm. All data are presented as mean ± SD (n = 3).
    Round Bottom 96 Well Plate, supplied by Beyotime, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/3d+cell+culture+coating+kit/pm39914543-192-8-15?v=Beyotime
    Average 99 stars, based on 1 article reviews
    round bottom 96 well plate - by Bioz Stars, 2026-08
    99/100 stars
    		  Buy from Supplier

    Image Search Results


    Cellular uptake and tumor spheroid penetration. (A) Confocal microscopy images of CT26-luc cells after 2 h of incubation with C6@LF NPs, C6@LFHA NPs, and competitive inhibition groups pre-treated with free LF or HA. Scale bar: 50 μm. (B) Flow cytometry histograms of (i) CT26-luc cells, (ii) human umbilical vein endothelial cells (HUVECs), and (iii) M2-polarized bone marrow-derived macrophages (M2-BMDMs) treated with C6@LF NPs, C6@LFHA NPs, and competitive inhibition groups. (C) Lysosomal co-localization of C6@LFHA NPs in HUVECs after 8 h incubation. Quantitative analysis showed a Pearson's correlation coefficient of 0.34 ± 0.026. (D) Penetration of C6@LFHA NPs into CT26-luc tumor spheroids, as shown by Z-stack slices (Scale bar: 20 μm), maximum intensity projection, and 3D reconstruction (generated using ImageJ). Scale bar: 200 μm. All data are presented as mean ± SD (n = 3).

    Journal: Bioactive Materials

    Article Title: LRP-1/CD44-targeted regorafenib nano-delivery system leveraging anti-angiogenesis and synergistic cytotoxicity against peritoneal metastasis of colorectal cancer

    doi: 10.1016/j.bioactmat.2025.12.015

    Figure Lengend Snippet: Cellular uptake and tumor spheroid penetration. (A) Confocal microscopy images of CT26-luc cells after 2 h of incubation with C6@LF NPs, C6@LFHA NPs, and competitive inhibition groups pre-treated with free LF or HA. Scale bar: 50 μm. (B) Flow cytometry histograms of (i) CT26-luc cells, (ii) human umbilical vein endothelial cells (HUVECs), and (iii) M2-polarized bone marrow-derived macrophages (M2-BMDMs) treated with C6@LF NPs, C6@LFHA NPs, and competitive inhibition groups. (C) Lysosomal co-localization of C6@LFHA NPs in HUVECs after 8 h incubation. Quantitative analysis showed a Pearson's correlation coefficient of 0.34 ± 0.026. (D) Penetration of C6@LFHA NPs into CT26-luc tumor spheroids, as shown by Z-stack slices (Scale bar: 20 μm), maximum intensity projection, and 3D reconstruction (generated using ImageJ). Scale bar: 200 μm. All data are presented as mean ± SD (n = 3).

    Article Snippet: For tumor spheroid penetration studies, CT26-luc multicellular tumor spheroids (MCTS) were prepared using a 3D cell culture coating kit (Beyotime).

    Techniques: Confocal Microscopy, Incubation, Inhibition, Flow Cytometry, Derivative Assay, Generated